Genome editing or genetic engineering:

CRISPR-Cas9 assay:

Gene editing technology, which provides precise modification of DNA sequences within the genome of living organisms. It is used to create complex cell-based assays that mimic disease states, enabling more physiologically relevant drug discovery and target validation

T7 endonuclease assay:

The T7 endonuclease assay is a used for evaluating the activity and efficiency of site-specific nucleases, such as the CRISPR-Cas9 system.

Ion Channel Assays:

Patch-clamp technique:

Measures ionic currents through individual ion channels.

Fluorescent dye-based assays:

Detect changes in ion concentrations (e.g., calcium, potassium).

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Reporter Gene Assays:

Luciferase reporter assay:

Measures luciferase enzyme activity as an indicator of gene expression.

β-galactosidase (LacZ) assay:

Uses the β-galactosidase enzyme as a reporter.

GFP/RFP (Green/Red Fluorescent Protein) assay:

Detects fluorescence as a marker for gene expression.


PCR

qPCR/qRT-PCR

ELISA

Western Blot