Genome editing or genetic engineering:
CRISPR-Cas9 assay:
Gene editing technology, which provides precise modification of DNA sequences within the genome of living organisms. It is used to create complex cell-based assays that mimic disease states, enabling more physiologically relevant drug discovery and target validation
T7 endonuclease assay:
The T7 endonuclease assay is a used for evaluating the activity and efficiency of site-specific nucleases, such as the CRISPR-Cas9 system.
Ion Channel Assays:
Patch-clamp technique:
Measures ionic currents through individual ion channels.
Fluorescent dye-based assays:
Detect changes in ion concentrations (e.g., calcium, potassium).
Reporter Gene Assays:
Luciferase reporter assay:
Measures luciferase enzyme activity as an indicator of gene expression.
β-galactosidase (LacZ) assay:
Uses the β-galactosidase enzyme as a reporter.
GFP/RFP (Green/Red Fluorescent Protein) assay:
Detects fluorescence as a marker for gene expression.
