Biochemical Assays
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- Biochemical Assays
Enzyme Activity Assays:
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Substrate-based assays
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Coupled enzyme assays
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Reporter enzyme assays
Binding Assays:
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Radioligand binding assays
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Fluorescence polarization (FP)
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Surface plasmon resonance (SPR)
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Isothermal titration calorimetry (ITC)
Protein-Protein Interaction Assays:
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Co-immunoprecipitation (Co-IP)
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Yeast two-hybrid (Y2H)
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Bioluminescence resonance energy transfer (BRET)
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Fluorescence resonance energy transfer (FRET)
Cell-based Assays:
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Reporter gene assays
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Cell viability assays
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Apoptosis assays
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High-content screening (HCS)
Receptor Binding Assays:
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GPCR (G-protein-coupled receptor) assays
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Ion channel assays
Nucleic Acid Assays:
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Quantitative PCR (qPCR)
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RNA interference (RNAi) assays
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CRISPR/Cas9-based assays
Post-translational Modification Assays
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Kinase assays
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Ubiquitination assays
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Methylation and acetylation assays
Metabolic Assays:
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Metabolite profiling
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Oxygen consumption rate (OCR)
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Extracellular acidification rate (ECAR)
Spectroscopic Assays:
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UV-Vis spectroscopy
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Mass spectrometry
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Nuclear magnetic resonance (NMR)
