OVA Induced Asthma Model

Asthma affects over 300 million people worldwide, presenting a major global health concern. Despite advances in treatment, there is still an urgent need for novel therapeutic approaches targeting the dominant pathways involved. At TheraIndx, we offer validated and reproducible OVA-induced asthma preclinical model to help accelerate your respiratory drug discovery process.

Harnessing the potential of OVA-induced asthma model for preclinical investigations

OVA-induced asthma is the most relevant model in order to study Th2 driven asthma. It effectively mirrors key pathophysiological features seen in human asthma, including:

• 1. Airway inflammation (Eosinophilic)
• 2. Th2 mediated
• 3. Airway hyperresponsiveness (AHR)
• 4. IgE mediated

How does the model work?

Mice are sensitised with OVA including an adjuvant (alum) on Day 0 and 7. On day 14, mice are challenged with OVA via multiple aerosol exposure resulting in Th2 mediated immune response. Our protocols are in-line with industry’s best practices and can be customised based on your requirements.

End points/ readouts

• • Effect on differential cell types in BALF
• • Serum OVA specific IgE levels
• • Cytokine profiling
• • Lung Histopathology

“Accelerate your Asthma therapeutics with confidence with TheraIndx”

References:

• 1. Kim, D.I., Song, MK. & Lee, K. Comparison of asthma phenotypes in OVA-induced mice challenged via inhaled and intranasal routes. BMC Pulm Med 19, 241 (2019).


• 2. Follettie, M., Ellis, D., Donaldson, D. et al. Gene expression analysis in a murine model of allergic asthma reveals overlapping disease and therapy dependent pathways in the lung. Pharmacogenomics J 6, 141–152 (2006).


• 3. Hanashiro, J., Muraosa, Y., Toyotome, T. et al. Schizophyllum commune induces IL-17-mediated neutrophilic airway inflammation in OVA-induced asthma model mice. Sci Rep 9, 19321 (2019).


• 4. Casaro M, Souza VR, Oliveira FA, Ferreira CM. OVA-Induced Allergic Airway Inflammation Mouse Model. Methods Mol Biol. 2019;1916:297-301. doi: 10.1007/978-1-4939-8994-2_28. PMID: 30535706.


• 5. Thakur VR, Khuman V, Beladiya JV, Chaudagar KK, Mehta AA. An experimental model of asthma in rats using ovalbumin and lipopolysaccharide allergens. Heliyon. 2019 Nov 19;5(11):e02864. doi: 10.1016/j.heliyon.2019.e02864. PMID: 31768443; PMCID: PMC6872797.